Stack Protocol

Best Peptides for Longevity: Stack and Doses 2026

NAD+ + Epitalon + GHK-Cu + Tesamorelin: four peptides targeting the four pillars of biological aging — mitochondrial function, telomere maintenance, systemic repair, and body composition.

Why These Four Peptides

NAD+ — Mitochondrial Fuel

Nicotinamide adenine dinucleotide. Central cofactor for mitochondrial ATP production and sirtuin activation. Declines with age. Restoring it has direct effects on cellular energy, DNA repair, and inflammation regulation.

Best for: Energy, mitochondrial function, sirtuin support

Route: IV infusion or subcutaneous

Epitalon — Telomere & Pineal Support

A 4-amino-acid peptide derived from pineal gland research. Activates telomerase in some cell lines, supports pineal melatonin signaling, and regulates the circadian-aging axis. Short cycles, large between-cycle gaps.

Best for: Telomere maintenance, melatonin axis, sleep architecture

Route: Subcutaneous, 10–20 day cycles

GHK-Cu — Systemic Repair

Copper tripeptide. Modulates inflammatory cytokines, drives connective tissue regeneration, and has gene expression effects on hundreds of repair-related pathways. The most visible early marker is skin quality, but systemic effects are the long-term goal.

Best for: Skin, connective tissue, inflammation

Route: Subcutaneous, daily

Tesamorelin — Body Composition

GHRH analog. Triggers a natural GH pulse, which over weeks reduces visceral adipose tissue (the most damaging fat depot for metabolic and inflammatory aging) while supporting muscle retention. Body composition is a longevity proxy.

Best for: Visceral fat, body composition, IGF-1 support

Route: Subcutaneous, at bedtime

Complementary Synergy: NAD+ fuels the energy and DNA repair machinery. Epitalon supports the telomere and circadian layer. GHK-Cu drives systemic repair. Tesamorelin shifts body composition into a more aging-resistant profile. Four mechanisms, one direction of travel.

Dosing Protocol

Peptide	Dose	Frequency	Timing	Duration
NAD+	100–250mg	2–3x weekly	IV or SC, morning	8–12 weeks, then maintain
Epitalon	5–10mg	Daily	Evening, SC	10–20 days, then 6-month gap
GHK-Cu	1–2mg	Daily	Evening, SC	4–8 weeks per cycle
Tesamorelin	1–2mg	Daily	At bedtime, SC	8 weeks on, 4 off

This is a 4-component stack. Most users start with 2 components and add the others once baseline labs and tolerance are established. Running all 4 from day one is not recommended.

Stack Variations by Goal

First-time longevity protocol

NAD+ + GHK-Cu only

Start with the two most established components. Run for 8 weeks, re-test, then layer in Epitalon and Tesamorelin.

Body composition focus

Tesamorelin + GHK-Cu + NAD+

Skip Epitalon. The first three components drive the most visible body composition and recovery changes.

Sleep + circadian repair

Epitalon + NAD+ + Tesamorelin

Drop GHK-Cu temporarily. Focus on the circadian axis. Pairs well with the Sleep Stack protocol.

Post-illness recovery (40+)

NAD+ + GHK-Cu (full stack later)

NAD+ rebuilds mitochondrial reserves. GHK-Cu modulates lingering inflammation. Add Tesamorelin once baseline strength returns.

Aesthetic longevity

GHK-Cu + Tesamorelin + NAD+

GHK-Cu skin effects + Tesamorelin body composition + NAD+ energy. The visible longevity stack.

Cellular aging focus (50+)

Full stack

All four components. Most aggressive protocol. Requires baseline labs and quarterly monitoring.

Longevity Response Timeline

Week 1–2

NAD+ effects (energy, clarity) usually emerge within the first 3–7 days of dosing. Epitalon and GHK-Cu effects are slower. Tesamorelin begins its body composition work.

Week 3–4

GHK-Cu starts to show on skin quality (the most visible early marker). Tesamorelin visceral fat reduction begins to be measurable. Sleep often improves indirectly.

Week 5–8

Mid-cycle. IGF-1 and inflammation markers should be re-tested. NAD+ users often shift to a maintenance frequency by this point.

Week 9–12

Plateau and re-evaluate. Continue the components that produced measurable change. Drop the components that did not.

Critical Points

Run baseline labs before starting. NAD+ and Tesamorelin both shift markers that you want to track. See the blood tests guide for the recommended panel.

NAD+ IV infusions take 1.5 to 3 hours. The infusion sensation (mild chest pressure, flushing) is normal and resolves quickly with slower drip rates.

Epitalon cycles are short by design. Do not run continuously. The 10–20 day pulses with 6-month gaps mirror the bioregulator dosing pattern.

GHK-Cu is the slowest-acting component on bloodwork but has the most visible early effect on skin and connective tissue.

Tesamorelin elevates IGF-1. If your baseline IGF-1 is already above mid-reference range, lower the dose or skip Tesamorelin entirely.

Refrigerate all reconstituted peptides. Most are stable for 3–4 weeks. NAD+ vials specifically should be used within 7 days of reconstitution.

Re-test mid-cycle (week 8 for an 8-week protocol). Drop components that show no movement. The longevity stack is data-driven or it is not real.

Cancer & Growth Caution:Tesamorelin raises IGF-1, GHK-Cu is pro-angiogenic, and Epitalon's telomerase effects are not fully characterized. Those with active cancer or significant cancer history should consult an oncologist before running this stack. NAD+ alone is the lowest-risk subset for cancer-cautious individuals.

NAD+ Guide Epitalon Guide GHK-Cu Guide Tesamorelin Guide Blood Tests in Vietnam NAD+ Therapy Guide