MOTS-c Reconstitution Calculator
Turn a target dose you already have into the exact injection volume and insulin syringe units for your vial. The math only. It never picks a dose for you.
Most people who search for a MOTS-c dosage calculator want a tool that tells them what to inject. This is not that. What number to run, and whether to run it at all, is a decision for a licensed prescriber who knows your history. This tool handles only the arithmetic: once you already have a target number from your own protocol or supplier, it converts that number into a concentration, a liquid volume in millilitres, and the units to draw on an insulin syringe. MOTS-c has no approved dose, and the sources on this page do not establish a human dose from an administered trial.
Enter your own numbers
The target dose starts at zero and stays there until you set it yourself, so nothing here is suggested to you. MOTS-c has no approved or established dose. Drag it to whatever your protocol source specifies.
Target dose from your protocol or supplier. The slider starts at zero and the results stay hidden until you move it off zero, so the page never puts a figure in front of you. MOTS-c has no established human dose. Drag it to the figure your own source specifies and the math updates as you go.
What the evidence and the community report
These are amounts recorded in published studies, a registry record, and one named community source, not recommendations. The Who column says whether a figure came from people or animals, and the Ref numbers point to the full citations in Sources below.
A. Approved label doses
None. No regulator has approved MOTS-c, so no label exists and the DailyMed label database returns zero records.
B. Amounts administered in published studies
No study administered MOTS-c itself by subcutaneous injection, the route this calculator assumes.
| Amount | Schedule | Route | Who | Ref |
|---|---|---|---|---|
| 15 mg/kg | twice daily | intraperitoneal | mouse, n not stated | [7] |
| not disclosed | single and multiple ascending dose | subcutaneous | human, n=88 | [8] |
No administered human MOTS-c amount was found: row two is CB4211, an analog, and its record names dose levels only.
C. Community reported practice
Self-reported, not peer reviewed, and the publisher earns commissions from the vendors it links to.
| Amount | Schedule | Route | Who | Ref |
|---|---|---|---|---|
| 5 mg | 2 to 3 times per week, for 4 to 8 weeks | subcutaneous | self-reported | [9] |
| 5 mg, then 5 mg, then 10 mg | twice weekly for 4 weeks, then three times weekly for 4 to 6 weeks, then 2 to 3 times weekly for 6 to 8 weeks | subcutaneous | self-reported | [9] |
Disclaimer: nothing here is a recommendation, animal figures are stated per kilogram of animal body weight and never convert into a human dose, and what to take is a decision for a licensed prescriber who knows your history.
Two worked math examples
Both examples use round numbers picked so the arithmetic is easy to follow. They are not recommendations and not doses to take. Use the figure your own protocol source specifies.
Example A: 10 mg vial with 2 mL
Math illustration, not a dose to take. The concentration is 10 / 2 = 5 mg/mL, or 5000 mcg/mL. A 5000 mcg target gives 5000 / 5000 = 1 mL, which is 100 units on a U-100 syringe. The vial contains 10000 / 5000 = 2 illustrative draws. On the 1.0 mL syringe selected for this illustration, the result exactly fills the barrel.
Example B: 10 mg vial with 2 mL
Math illustration, not a dose to take. The concentration stays at 5 mg/mL, or 5000 mcg/mL. A 1000 mcg target gives 1000 / 5000 = 0.2 mL, which is 20 units on a U-100 syringe. The vial contains 10000 / 1000 = 10 illustrative draws. The result fits the 0.5 mL syringe selected for this illustration.
The vial amount and water volume fix the concentration. Changing the entered target number changes the liquid volume and syringe reading. Enter your own numbers above to run the same calculation.
The calculator's scope and boundary
A reconstitution calculator answers a mechanical question. You have a vial of freeze dried peptide and add a known volume of water. That fixes the concentration. From there, any target number you enter has one matching draw volume, and the calculator finds it. There is no judgment in that step, only division.
The calculator will not choose a dose. A tool that reads your weight or goals and hands back "inject this much" is giving medical advice, and this calculator does not do that. The target number is something you bring to the calculator, not something it gives you. If you do not have a number from a licensed prescriber or a protocol source you trust, no calculation on this page can supply one.
Reconstituting the vial
If your vial contains lyophilized powder, reconstitution means adding the water volume you entered above and letting the powder dissolve. These are general vial-handling steps, not instructions for choosing or administering a dose.
- Wipe both rubber stoppers, the peptide vial and the water vial, with an alcohol swab and let them dry.
- Draw the water volume you entered in the calculator into a syringe.
- Insert the needle at a slight angle so the water runs down the inside wall of the vial rather than directly onto the powder.
- Inject the water slowly. Do not shake the vial. Swirl gently until the solution is clear.
- Label the vial with the date and the calculated concentration in mg/mL.
- Follow the supplier's written storage and handling instructions.
Water choice and storage sit outside the calculator's arithmetic. For the difference between the two common water types, see bacteriostatic water vs sterile water. For a general mixing walkthrough, see peptide mixing basics.
Picking the BAC water volume
The amount of water you add does not change how much peptide is in the vial. It changes the concentration and therefore the liquid volume for the target number you enter. A 10 mg vial reconstituted with 1 mL is twice as concentrated as the same vial with 2 mL, so the draw is half the volume for the same target. More water spreads the syringe marks out, while less water concentrates the draw. The calculator recomputes instantly so you can compare the arithmetic with the written instructions that came with your vial.
Reading the insulin syringe
The calculator uses the U-100 conversion:
1 unit = 0.01 mL
100 units = 1 mL = full 1 mL barrel
A result of 0.5 mL is 50 units, and a result of 0.25 mL is 25 units. The calculator shows both formats so you can match the liquid volume to a U-100 syringe. It also checks the result against the selected 0.3 mL, 0.5 mL, or 1.0 mL barrel capacity.
Why the calculator does not choose a dose
The listed papers cover mouse and cell studies, measurements of the MOTS-c made by the human body, and a review of mitochondrial-derived peptides. They do not establish an approved human dose or a published human dose-finding result for administered MOTS-c. Those sources cannot give a calculator a basis for selecting a target number.
The sources explain that boundary. They are not here to turn this page into a compound explainer. For information about the compound itself, read the MOTS-c compound profile.
Sources
1. Lee C, Zeng J, Drew BG, Sallam T, Martin-Montalvo A, Wan J, et al. The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance. Cell Metab. 2015 Mar 3;21(3):443-54. PMID: 25738459. DOI: 10.1016/j.cmet.2015.02.009.
2. Reynolds JC, Lai RW, Woodhead JST, Joly JH, Mitchell CJ, Cameron-Smith D, et al. MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis. Nat Commun. 2021 Jan 20;12(1):470. PMID: 33473109. DOI: 10.1038/s41467-020-20790-0.
3. Du C, Zhang C, Wu W, Liang Y, Wang A, Wu S, et al. Circulating MOTS-c levels are decreased in obese male children and adolescents and associated with insulin resistance. Pediatr Diabetes. 2018 Apr 25. PMID: 29691953. DOI: 10.1111/pedi.12685.
4. Ramanjaneya M, Jerobin J, Bettahi I, Bensila M, Aye M, Siveen KS, et al. Lipids and insulin regulate mitochondrial-derived peptide (MOTS-c) in PCOS and healthy subjects. Clin Endocrinol (Oxf). 2019 Aug;91(2):278-287. PMID: 31066084. DOI: 10.1111/cen.14007.
5. Yang B, Yu Q, Chang B, Guo Q, Xu S, Yi X, et al. MOTS-c interacts synergistically with exercise intervention to regulate PGC-1α expression, attenuate insulin resistance and enhance glucose metabolism in mice via AMPK signaling pathway. Biochim Biophys Acta Mol Basis Dis. 2021 Jun 1;1867(6):166126. PMID: 33722744. DOI: 10.1016/j.bbadis.2021.166126.
6. Merry TL, Chan A, Woodhead JST, Reynolds JC, Kumagai H, Kim SJ, et al. Mitochondrial-derived peptides in energy metabolism. Am J Physiol Endocrinol Metab. 2020 Oct 1;319(4):E659-E666. PMID: 32776825. DOI: 10.1152/ajpendo.00249.2020.
7. MOTS-c partially protects against skeletal muscle deterioration in C26 cachexia. Front Med (Lausanne). 2026. PMID: 42266945. Mouse, male, Colon-26 carcinoma cachexia model; n not stated in the abstract. The figure is stated per kilogram of animal body weight and no human equivalent is provided by the paper or derivable from it.
8. A Phase 1a/1b Study of CB4211, sponsor CohBar Inc, status COMPLETED. ClinicalTrials.gov registry record NCT03998514. Human, healthy non-obese subjects and subjects with nonalcoholic fatty liver disease, 88 enrolled per the record. CB4211 is a MOTS-c ANALOG, not MOTS-c itself, and the registry record labels the dose levels only as Dose 1 through Dose 6, across six single ascending dose levels plus multiple ascending dose groups, so no amount can be read from it even for the analog.
9. Peptide Dosing Protocols (Garret Grant). MOTS-c Peptide Dosing Guide. https://www.peptidedosingprotocols.com/protocol/mots-c. Fetched 2026-08-04. Self-reported, not peer reviewed, a self-selected community rather than a study population, so no n applies. Commercial interest, stated plainly: the publisher does not sell peptides directly but earns commissions from the vendors it links to, and states of its own accord that none of these tiers has been validated in a published human randomized trial and that no published human randomized controlled trial has tested native MOTS-c for any clinical endpoint.
Frequently asked questions
Is there an established MOTS-c dose for this calculator?
No. MOTS-c is not FDA-approved for any use, so there is no label dose, and the sources listed on this page do not establish a human dose from an administered trial. The target dose starts at zero and the calculator never sets a figure for you. Whatever number you drag it to has to come from your own licensed prescriber or a protocol you trust.
How do I use this calculator for my own target dose?
The target dose starts at zero and waits for your own number. Drag the dose slider to whatever figure your own protocol or supplier specifies, in mcg or mg, and the calculator recomputes the injection volume, insulin syringe units, and how many doses the vial holds. It never judges the number you enter. For the same arithmetic without a compound preset, use the general peptide reconstitution calculator.
How much BAC water do I add to a 10 mg MOTS-c vial?
There is no single answer from the calculator because water volume changes concentration and syringe readability, not the amount of peptide in the vial. A 10 mg vial in 2 mL gives 5 mg/mL. The same vial in 1 mL gives 10 mg/mL, so the same target number draws half the liquid volume. Enter the water volume from your written protocol or supplier instructions and the calculator will show the resulting draw.
Why does this calculator not choose a target dose?
A reconstitution calculator can divide the peptide in a vial by the water added, but it cannot turn animal studies, cell work, or observations about the MOTS-c made by the human body into medical advice. The cited sources do not establish a human dose for administered MOTS-c. You bring the target number, and the calculator handles only the concentration and draw math.
Why does changing BAC water change the syringe reading?
The amount of peptide in the vial stays fixed. Adding more water lowers the concentration, so the same target number needs a larger draw volume. Adding less water raises the concentration, so the same target number needs a smaller draw volume. The calculator updates both millilitres and U-100 syringe units when you move the water slider.
How do I read insulin syringe units?
On a U-100 insulin syringe, 1 unit equals 0.01 mL and 100 units equals 1 mL. The calculator multiplies the liquid volume in millilitres by 100 and shows the result as syringe units. Check that result against the markings and capacity of the syringe you selected.
Is this calculator medical advice?
No. It is harm reduction education. It performs reconstitution arithmetic on numbers you supply. It does not diagnose, does not prescribe, and does not tell you what dose to take. Dose and schedule are a prescriber decision. Always cross-check with your protocol source, supplier instructions, and where possible a qualified clinician.
This is harm reduction education, not medical advice
This calculator is for educational use. It converts a dose you already have into a volume and syringe reading. It does not choose a dose. Peptide protocols vary between individuals and compounds. Always cross-check with your protocol source, supplier instructions, and where possible a qualified clinician.